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Can structural insights to ligase system and substrate specificity be exploited to build novel catalysts, kinds that should support in comprehending the genetics and mobile biology of DNA maintenance and in the observe of enzymatic joining and synthesis of nucleic acids? There is sweet motive to Imagine so. Character has by now confounded the partitioning of ligases into rigid DNA-distinct and RNA-distinct clades. We now take pleasure in a large number of DNA ligases are adept at sealing substrates that contains an all-RNA 3′-OH strand (eight, 25, 26) Which some RNA ligases readily seal molecules with as couple as two or 3 ribonucleotides at the three′-OH terminus within an normally all-DNA context (27, 28).
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